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In practice, there are sufficient variations in these conditions that it is necessary to correct a sample’s raw radiocarbon age by comparing it with the standard calibration curve.
Carbon-14 dating depends on the amount of atmospheric carbon-14 relative to carbon-12.
A final assumption is that the various rates of flow of carbon-14 among the respective geophysical reservoirs must be constant, and the residence time of carbon-14 in the various reservoirs must be short relative to its half-life. (Riverside, CA: La Sierra University Press, 1997), 183. Carbon-14 dating models and experimental implications. Origins 24(2, 1997):50-64  The deep ocean waters are estimated to take about 1000 years to circulate back to the surface: Aitken, MJ.
If these three conditions are met, the initial concentration of C in the sample can be estimated.
These effects are corrected for by comparing samples from different locations. C in coal and oil is much less than in the present environment. This gives a ratio of 300:1 for pre-Flood biosphere carbon to present biosphere carbon. If a significant portion of the coal and oil represents organisms that were deposited in a global flood, then the pre-flood atmosphere must have contained much less C over time resulting in wobbles and deviation from expected levels in the standard calibration curve based on samples of known age?  Note the wobbles in the curves in Pearson, GW et al. Evidence for correlations between nuclear decay rates and Earth-Sun distance – et al. This differs by a factor of only two from the figure of 143:1 proposed by Brown (Brown, RH. Violations of this assumption can frequently be identified.Three additional assumptions are necessary in radiocarbon dating in order to estimate the initial concentration of C could be caused by changes in the intensity of the cosmic radiation or in the strength of the earth’s magnetic field.