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This excludes the latter as a potential ancestral source population for early European farmers and suggests a strong genetic structure in hunter-gatherers of Southwest Asia. Ancient samples are abbreviated consistently using the nomenclature “site-country code-culture”; see and Figs.

S8–S10; Dataset S2); ‡Khomani San were selected as an outgroup as they are considered to be the most genetically diverged extant human population.

Farming and sedentism first appeared in southwestern Asia during the early Holocene and later spread to neighboring regions, including Europe, along multiple dispersal routes.We use a novel approach to recalibrate raw reads and call genotypes from ancient DNA and observe striking genetic similarity both among Aegean early farmers and with those from across Europe.Our study demonstrates a direct genetic link between Mediterranean and Central European early farmers and those of Greece and Anatolia, extending the European Neolithic migratory chain all the way back to southwestern Asia.All these sites show material culture affinities with the central and southwestern Anatolian Neolithic (6).Early Greek Neolithic sites, such as the Franchthi Cave in the Peloponnese, Knossos in Crete, and Mauropigi, Paliambela, and Revenia in northern Greece date to a similar period (7–9).

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